Racked Optical Sensitive PCR Sealing Film
1. Common PCR sealing film.
Suitable for PCR reactions, material is polypropylene membrane
RNase/DNase and nucleic acid free
Easy to seal the plate, not easy to curl
Working temperature -40℃- +120℃ can be operated
2. Fluorescent PCR sealing film.
Transparent, low autofluorescence interference, suitable for fluorescent quantitative PCR experiments
Suitable for all kinds of PCR plates, not a puncture membrane
DNase/RNase and nucleic acid free, anti-DMSO
Easy to seal, not easy to curl
Working temperature -70℃--+100℃
3. PCR aluminium sealing film
Non-permeable soft aluminium film with medical grade adhesive, suitable for long term storage of samples
Compared to other aluminium sealing films, this film is less prone to curling,
Excellent anti-evaporation properties, virtually no evaporation of samples, easy to puncture
DNase/RNase and nucleic acid free
Suitable for all types of PCR plates, including those with raised edges
4. Microplate permeable sealing film
|Name||Racked Optical Sensitive PCR Sealing Film|
|Application||PCR, qPCR, ELISA, cell culture, long-term storage, automated workstation processing|
|Specification||100 pieces / case|
|Lead time||According to the order quantity, the lead time is about 1-7 days.|
|Shipping||DHL, UPS, TNT, FEDEX, Air/Sea Freight|
|Payment||Paypal, T/T, L/C, Western Union, etc.|
1. Remove the single sheet of sealing film or sealing foil from the self-sealing bag and reseal the bag to maintain the enzyme-free environment within.
2. Hold the sealing film or foil with the backing side up. Fold the end tab down at the tangent line of the backing.
3. If the product being used is a single end-labelled sealing film or foil, partially remove the liner, then anchor the sealing film or foil to the board so that it is sealed over the entire board and proceed to remove the liner. This method eliminates curling and rollback from the sealing film or foil.
4. If using a product with two end tabs, peel the centre liner in a continuous smooth manner. Slow peeling of the liner will reduce curling to a large extent. Take care not to touch the adhesive surface of the film.
5. Using both hands, grasp the white part of the two ends and lower the film onto the aperture plate.
6. Use the platen to slowly scrape and press the sealing film or foil to seal it to the plate. This step should be performed at least twice, horizontally and vertically. Applying sufficient force is essential to obtain a good seal.
7. Scrape the platen along all outer edges of the perforated plate at least twice to ensure firm and consistent pressure is applied.
8. Inspect the plate after sealing to confirm a tight bond between the film/foil and the plate.
Note: The optical sealing film or foil should not be wrinkled; if wrinkles are observed, the plate has not been sealed correctly. Also note that the sealing film or foil should not extend upwards onto the side walls of the plate. This may occur with flat panels with raised edges due to incorrect positioning of the sealing film or foil on the panel, or if the joints at the ends have not been removed. Verify that the paste marks around each hole and that the entire surface of the plate (including the perimeter) is sealed.